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prime script tm rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script tm rt reagent kit
    Prime Script Tm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88818 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prime+script+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pmc13616764-84-14-20
    Average 99 stars, based on 88818 article reviews
    prime script tm rt reagent kit - by Bioz Stars, 2026-10
    99/100 stars

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    Reverse Transcription:

    Article Title: The protective role of a moderate protein diet in ETEC-infected piglets: Optimization of growth, immunity, and microbial balance
    Article Snippet: Total RNA was extracted from jejunum and ileal mucosa using the Trizol Reagent (TaKaRa Biotechnology Co., Ltd., Dalian, Liaoning, China), and the concentration and purity of total RNA were assayed by spectrophotometer (NanoDrop, Gene Co., Ltd., Guangzhou, Guangdong, China) at 260 and 280 nm according to the manufacturer’s instructions. .. Reverse transcription was carried out using the Prime Script RT reagent kit (TaKaRa Biotechnology Co., Ltd., Dalian, Liaoning, China) according to the manufacturer’s instructions. .. Quantitative real-time PCR was performed to analyze the expression levels of IL-1β , IFN-γ , IL-6 , Bax , Bcl-2 , and C aspase-3 using TB Green Premix Ex Taq II Fast qPCR reagent (TaKaRa Biotechnology Co., Ltd., Dalian, Liaoning, China) and the QuanStudio 6 Flex Real-Time PCR detection system (Applied Biosystems Inc., Foster City, CA, USA).

    Article Title: Exosomes from bone marrow mesenchymal stem cells inhibit osteoclast differentiation and alleviate osteoporosis via RBM15B/YAP1 to induce autophagy.
    Article Snippet: Background: Osteoporosis develops primarily as a result of an imbalance between osteoclastic bone resorption and osteoblastic bone formation.. Bone marrow mesenchymal stem cells-derived-exosomes (BMSCs-Exos) regulate osteoclast differentiation and osteoporosis in recent studies.. But the mechanisms are still unclear.

    Article Title: Bitter pit disorder development: Evidence from transcriptomic and cellular analysis in different tissues.
    Article Snippet: Bitter pit (BP), a physiological disorder, causes significant losses to the apple industry.. The precise mechanisms underlying BP remain incompletely understood.. Herein, transcriptomic analysis was carried out on healthy flesh (FH), flesh between healthy and disordered (FB), disordered flesh (FD), healthy pericarp (PH), and disordered pericarp (PD).

    Article Title: Omega-3 PUFAs Mitigate Polytrauma-Induced Gut-Liver-Lung Inflammation and Organ Dysfunction via GPR120/PPAR-γ
    Article Snippet: RNA purity and concentration were verified via a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), with strict inclusion criteria of an A260/A280 ratio between 1.8 and 2.0. .. Subsequently, 1 μg of RNA was reverse-transcribed into cDNA utilizing the Prime Script RT reagent Kit (Takara Bio, Shiga, Japan). .. Quantitative PCR was executed on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using the SYBR Green (Vazyme Biotech, Nanjing, China).

    Article Title: High‑fat diet exacerbates hepatic and adipose insulin signaling impairment and ovarian lesions in a rat model of letrozole‑induced polycystic ovary syndrome.
    Article Snippet: Histological criteria Score Steatosis <5% 0 5‐33% 1 >34‐66% 2 >66% 3 Inflammation no foci 0 <2 foci/x200 1 2‐4 foci/x200 2 >4 foci/x200 3 Hepatocyte ballooning none 0 Few balloon cells 1 Prominent ballooning 2 Fibrosis stage none 0 Perisinusoidal or periportal 1 Perisinusoidal and portal/periportal 2 Bridging fibrosis 3 cirrhosis 4 using easy Blue reagent (intron Biotechnology, Seongnam, Korea) according to the manufacturer's protocol. .. For each sample, 5 μg of rna was reverse transcribed into cdna using the Prime Script rT reagent Kit (TaKara Biotech, dalian, china), and quantitative Pcr was performed using TB Green Pcr Master Mix (TaKara Biotech, dalian, china). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Exosomes from bone marrow mesenchymal stem cells inhibit osteoclast differentiation and alleviate osteoporosis via RBM15B/YAP1 to induce autophagy.
    Article Snippet: Background: Osteoporosis develops primarily as a result of an imbalance between osteoclastic bone resorption and osteoblastic bone formation.. Bone marrow mesenchymal stem cells-derived-exosomes (BMSCs-Exos) regulate osteoclast differentiation and osteoporosis in recent studies.. But the mechanisms are still unclear.

    Article Title: Bitter pit disorder development: Evidence from transcriptomic and cellular analysis in different tissues.
    Article Snippet: Bitter pit (BP), a physiological disorder, causes significant losses to the apple industry.. The precise mechanisms underlying BP remain incompletely understood.. Herein, transcriptomic analysis was carried out on healthy flesh (FH), flesh between healthy and disordered (FB), disordered flesh (FD), healthy pericarp (PH), and disordered pericarp (PD).

    Article Title: High‑fat diet exacerbates hepatic and adipose insulin signaling impairment and ovarian lesions in a rat model of letrozole‑induced polycystic ovary syndrome.
    Article Snippet: Histological criteria Score Steatosis <5% 0 5‐33% 1 >34‐66% 2 >66% 3 Inflammation no foci 0 <2 foci/x200 1 2‐4 foci/x200 2 >4 foci/x200 3 Hepatocyte ballooning none 0 Few balloon cells 1 Prominent ballooning 2 Fibrosis stage none 0 Perisinusoidal or periportal 1 Perisinusoidal and portal/periportal 2 Bridging fibrosis 3 cirrhosis 4 using easy Blue reagent (intron Biotechnology, Seongnam, Korea) according to the manufacturer's protocol. .. For each sample, 5 μg of rna was reverse transcribed into cdna using the Prime Script rT reagent Kit (TaKara Biotech, dalian, china), and quantitative Pcr was performed using TB Green Pcr Master Mix (TaKara Biotech, dalian, china). ..

    Isolation:

    Article Title: Phenotyping of TH9 Cells in Cytomegalovirus-reactivated Kidney Transplant Recipients.
    Article Snippet: .. Total RNA was extracted from the isolated CD4+ T cells using TRIzol reagent, and cDNA synthesis was carried out using the Prime Script RT Reagent Kit (Takara, Japan), following the manufacturer’s recommendations. .. Realtime PCR reactions were conducted using the ABI Step One Plus instrument (Applied Biosystems Step One plus, USA).

    cDNA Synthesis:

    Article Title: Phenotyping of TH9 Cells in Cytomegalovirus-reactivated Kidney Transplant Recipients.
    Article Snippet: .. Total RNA was extracted from the isolated CD4+ T cells using TRIzol reagent, and cDNA synthesis was carried out using the Prime Script RT Reagent Kit (Takara, Japan), following the manufacturer’s recommendations. .. Realtime PCR reactions were conducted using the ABI Step One Plus instrument (Applied Biosystems Step One plus, USA).

    Article Title: LINC00324 induces nucleus pulposus cell apoptosis in intervertebral disc degeneration via miR-143-3p targeted TRAIL/DR4/DR5 axis
    Article Snippet: Total RNA was isolated from tissues and cells using miRNeasy Mini Kit (Qiagen, Hilden, Germany) or TRIzol reagent (Takara, Dalian, Japan). .. Then, RNA was transcribed into cDNA using miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (Vazyme, Nanjing, China) or Prime Script RT Reagent Kit (TaKaRa, Dalian, Japan). ..

    Quantitative RT-PCR:

    Article Title: Bitter pit disorder development: Evidence from transcriptomic and cellular analysis in different tissues.
    Article Snippet: Bitter pit (BP), a physiological disorder, causes significant losses to the apple industry.. The precise mechanisms underlying BP remain incompletely understood.. Herein, transcriptomic analysis was carried out on healthy flesh (FH), flesh between healthy and disordered (FB), disordered flesh (FD), healthy pericarp (PH), and disordered pericarp (PD).

    SYBR Green Assay:

    Article Title: Bitter pit disorder development: Evidence from transcriptomic and cellular analysis in different tissues.
    Article Snippet: Bitter pit (BP), a physiological disorder, causes significant losses to the apple industry.. The precise mechanisms underlying BP remain incompletely understood.. Herein, transcriptomic analysis was carried out on healthy flesh (FH), flesh between healthy and disordered (FB), disordered flesh (FD), healthy pericarp (PH), and disordered pericarp (PD).



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