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prime script rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script rt reagent kit
    Prime Script Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prime+script+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pmc13101298-80-6-11
    Average 99 stars, based on 88394 article reviews
    prime script rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Cadherin-11 serves as a novel receptor for Fusobacterium nucleatum adhesin FadA to exacerbate pulmonary inflammation.
    Article Snippet: .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) Total RNA was extracted from A549 cells using RNAiso Plus (Takara, China), and reversely transcribed into complementary DNA using Prime Script RT Reagent Kit (Takara, China). .. QRT-PCR analyses were performed with SYBR Green Master Mix (Takara, China) on an Applied Biosystems 7500 Real‐Time PCR System (Waltham, MA, USA).

    Reverse Transcription:

    Article Title: The protective role of a moderate protein diet in ETEC-infected piglets: Optimization of growth, immunity, and microbial balance
    Article Snippet: Total RNA was extracted from jejunum and ileal mucosa using the Trizol Reagent (TaKaRa Biotechnology Co., Ltd., Dalian, Liaoning, China), and the concentration and purity of total RNA were assayed by spectrophotometer (NanoDrop, Gene Co., Ltd., Guangzhou, Guangdong, China) at 260 and 280 nm according to the manufacturer’s instructions. .. Reverse transcription was carried out using the Prime Script RT reagent kit (TaKaRa Biotechnology Co., Ltd., Dalian, Liaoning, China) according to the manufacturer’s instructions. .. Quantitative real-time PCR was performed to analyze the expression levels of IL-1β , IFN-γ , IL-6 , Bax , Bcl-2 , and C aspase-3 using TB Green Premix Ex Taq II Fast qPCR reagent (TaKaRa Biotechnology Co., Ltd., Dalian, Liaoning, China) and the QuanStudio 6 Flex Real-Time PCR detection system (Applied Biosystems Inc., Foster City, CA, USA).

    Article Title: Innovative 3D-Image Analysis of Cerebellar Vascularization Highlights Angiogenic Gene Dysregulations in a Murine Model of Apnea of Prematurity
    Article Snippet: .. Messenger RNAs were converted to cDNA by reverse transcription using the Prime Script RT reagent kit (cat. RR037A, Takara). ..

    Article Title: Transcriptome profile of keloid patients in Zunyi, China via multiple bioinformatic analyses.
    Article Snippet: Background: Keloids are pathological scars.. There is a genetic predisposition for keloids, and the molecular events associated with keloid need to be profiled.. Methods: This study used whole-genome RNA-Seq to analyze aberrant gene expression between 17 keloid and 8 normal skin samples at the Affiliated Hospital of Zunyi Medical University, followed by comprehensive bioinformatic analysis.

    Article Title: Bitter pit disorder development: Evidence from transcriptomic and cellular analysis in different tissues.
    Article Snippet: Bitter pit (BP), a physiological disorder, causes significant losses to the apple industry.. The precise mechanisms underlying BP remain incompletely understood.. Herein, transcriptomic analysis was carried out on healthy flesh (FH), flesh between healthy and disordered (FB), disordered flesh (FD), healthy pericarp (PH), and disordered pericarp (PD).

    Article Title: FOXO3 Regulated Granulosa Cells Apoptosis and AKT1 Phosphorylation in Muscovy Duck Follicles (Cairina moschata)
    Article Snippet: The total RNA was extracted using TRIzol Reagent (TIANGEN, China) following the standard protocol (Roy et al., 2020). .. RNA purity was assessed prior to reverse transcription, which was carried out with the Prime Script RT reagent Kit with gDNA Eraser (Takara, Japan). .. RT-qPCR was performed using SYBR Green Master Mix (Applied Biosystems, US) on an ABI 7500 system under the following conditions: 95°C for 2 min; 40 cycles of 95°C for 15 s, 60°C for 30 s, and 72°C for 30 s. Gene expression was normalized to GAPDH (Feng et al., 2024) and calculated via the 2-△△Ct method(Schmittgen and Livak, 2008).

    Polymerase Chain Reaction:

    Article Title: Transcriptome profile of keloid patients in Zunyi, China via multiple bioinformatic analyses.
    Article Snippet: Background: Keloids are pathological scars.. There is a genetic predisposition for keloids, and the molecular events associated with keloid need to be profiled.. Methods: This study used whole-genome RNA-Seq to analyze aberrant gene expression between 17 keloid and 8 normal skin samples at the Affiliated Hospital of Zunyi Medical University, followed by comprehensive bioinformatic analysis.

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptome profile of keloid patients in Zunyi, China via multiple bioinformatic analyses.
    Article Snippet: Background: Keloids are pathological scars.. There is a genetic predisposition for keloids, and the molecular events associated with keloid need to be profiled.. Methods: This study used whole-genome RNA-Seq to analyze aberrant gene expression between 17 keloid and 8 normal skin samples at the Affiliated Hospital of Zunyi Medical University, followed by comprehensive bioinformatic analysis.

    Article Title: Bitter pit disorder development: Evidence from transcriptomic and cellular analysis in different tissues.
    Article Snippet: Bitter pit (BP), a physiological disorder, causes significant losses to the apple industry.. The precise mechanisms underlying BP remain incompletely understood.. Herein, transcriptomic analysis was carried out on healthy flesh (FH), flesh between healthy and disordered (FB), disordered flesh (FD), healthy pericarp (PH), and disordered pericarp (PD).

    Quantitative RT-PCR:

    Article Title: Bitter pit disorder development: Evidence from transcriptomic and cellular analysis in different tissues.
    Article Snippet: Bitter pit (BP), a physiological disorder, causes significant losses to the apple industry.. The precise mechanisms underlying BP remain incompletely understood.. Herein, transcriptomic analysis was carried out on healthy flesh (FH), flesh between healthy and disordered (FB), disordered flesh (FD), healthy pericarp (PH), and disordered pericarp (PD).

    SYBR Green Assay:

    Article Title: Bitter pit disorder development: Evidence from transcriptomic and cellular analysis in different tissues.
    Article Snippet: Bitter pit (BP), a physiological disorder, causes significant losses to the apple industry.. The precise mechanisms underlying BP remain incompletely understood.. Herein, transcriptomic analysis was carried out on healthy flesh (FH), flesh between healthy and disordered (FB), disordered flesh (FD), healthy pericarp (PH), and disordered pericarp (PD).



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